Fatty acids profile in fish plasma
Pages 56-62
A. Iordache, E. Horj, A. R. Ani, C. Mesaros, S. Morar, M. Culea
Abstract. Eicosapentaenoic acid (EPA) and docosapentaenoic acid (DHA) are the major fatty acids found in fish. These fatty acids are produced by unicellular algae and phytoplankton which are consumed and then accumulate in fish. [1-3]. The aim of this work was to establish the extraction procedure, the derivatization method, the separation temperature program and the identification of the fatty acids from fish plasma. The extraction of the fatty acids was performed by mixing plasma and chloroform:methanol (2:1) during 30 seconds, at room temperature. The identification of fatty acids was obtained by comparison of FAME mass spectra with the mass spectra of fatty acids methyl esters (FAME) kits and of NIST library.